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Please use this identifier to cite or link to this item: http://hdl.handle.net/1889/3750

Title: Selective adsorption of functional fibronectin through aptamer functionalization: a new concept for tailoring materials bioactivity
Authors: Parisi, Ludovica
Issue Date: Mar-2019
Publisher: Università degli Studi di Parma. Dipartimento di Medicina e chirurgia
Document type: Doctoral thesis
Abstract: Protein adsorption on biomaterials occurs shortly after scaffold insertion and it is of pivotal importance to address cell-material interaction and to achieve therapeutic success in tissue engineering. Therefore, its control may represent a crucial node in the design of implantable devices. In this thesis, aptamers, which are short oligonucleotides able to recognize, bind and retain target proteins with high affinity, have been identified as an innovative cue to address the selective adsorption of fibronectin from plasma at chitosan interface. Selective fibronectin chitosan surfaces were prepared and characterized for the amelioration of fibronectin adsorption both quantitatively and qualitatively. Furthermore, cell response was studied against murine osteoblastic-like MC3T3-E1 cells and against human epithelial-like HeLa cells. The results we obtained supports the use of aptamers as a new viable modification to improve the adsorption of specific proteins at scaffold interface and to promote the response of cells in term of adhesion, spreading, migration and proliferation. To conclude, we believe to be at the eve of a new promising way to tailor scaffold biocompatibility and to design new targeted and customized therapies in tissue engineering.
Appears in Collections:Medicina molecolare, tesi di dottorato

Files in This Item:

File Description SizeFormatVisibility
Finale - Parisi Ludovica.pdfTesi di dottorato8,98 MBAdobe PDFavailable from: 1/4/2020
Final Report Activities - Parisi Ludovica.pdfRelazione finale attività di dottorato3,26 MBAdobe PDFNot available

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