DSpaceUnipr DSpaceUnipr
      Le Biblioteche dell'Università    |    Periodici elettronici    |    Banche dati    |    Cataloghi online

DSpace a Parma >
Tesi di dottorato e di master >
Medicina molecolare, tesi di dottorato >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1889/3748

Title: An experimental 3D model of the rat, thyroid gland stromal scaffold: implications for bioengineering of a bioartificial thyroid gland
Other Titles: Un modello sperimentale 3D della matrice stromale della ghiandola tiroide di ratto: implicazioni per la bioingegneria di una tiroide bioartificiale
Authors: Alfieri, Marco
Issue Date: Mar-2019
Publisher: Università degli Studi di Parma. Dipartimento di Medicina e chirurgia
Document type: Doctoral thesis
Abstract: My PhD project aims at characterizing an ex situ model of adult male rat thyroid gland, recently designed and experimentally developed at the University of Parma, Parma, Italy by the research group headed by Prof. Roberto Toni MD PhD, and chosen as a key experimental tool for the activity of the recently funded, five-years Horizon 2020 SCREENED research project (Coordinator, Prof. Lorenzo Moroni, PhD, University of Maastricht, NL) . According to the principles of regenerative medicine and tissue engineering, we have selected a combination of biocompatible / bioerodible materials to be used as 3D scaffold substrates, and adult rat thyroid stem cells induced to differentiation to give rise to a 3D thyroid organoid, grown in either static or dynamic bioreactor-like cell culture. In the first year of my PhD program, I have characterized the stromal composition of the decellularized adult male rat thyroid lobe (i.e. its matrix) using two different mass spectrometry approaches coupled to appropriate bioinformatics software. In the second year of my PhD program, I have investigated the use of collagen from rat tail tendons and polysaccharide substances like chitosan as chemical substrates suitable for bioprinting technology. Under the supervision of Prof. Lisa Elviri PhD at the Department of Food Science and Pharmacology of the University of Parma, Parma, Italy I have in vitro engineered 3D reticular structures, geometrically and chemically consistent with parts of the stromal matrix of the rat thyroid lobe, to be used as scaffolds for thyroid stem cell homing. In the third year of my PhD program, in collaboration with the Proteomic Group of the University College of Dublin (Ireland) leaded by Prof. Stephen R. Pennington PhD, I have conducted a study leading to the proteomic signature of adult male rat thyroid stem cell / progenitors. These results will be exploited to identify molecular makers for selective sorting by cytometric technology of adult male rat thyroid stem cells directly from their native tissue, and to enlighten still uncovered biological properties of these cell precursors.
Appears in Collections:Medicina molecolare, tesi di dottorato

Files in This Item:

File Description SizeFormatVisibility
Marco Alfieri - Tesi dottorato XXXI ciclo Medicina Molecolare.pdfTesi di dottorato4,99 MBAdobe PDFavailable from: 1/4/2021
Marco Alfieri - relazione attività svolte.pdfRelazione finale attività di dottorato301,75 kBAdobe PDFNot available


This item is protected by original copyright



Items in DSpaceUnipr are protected by copyright, with all rights reserved, unless otherwise indicated.


Share this record
Del.icio.us

Citeulike

Connotea

Facebook

Stumble it!

reddit


 

  Browser supportati Firefox 3+, Internet Explorer 7+, Google Chrome, Safari

ICT Support, development & maintenance are provided by CINECA. Powered on DSpace Software
CINECA